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[size=1.0625]ACCEPTED MANUSCRIPT
Serosurvey for SARS-CoV-2 among blood donors in Wuhan, China from September to December 2019
[url=]Le Chang[/url], [url=]Lei Zhao[/url], [url=]Yan Xiao[/url], [url=]Tingting Xu[/url], [url=]Lan Chen[/url], [url=]Yan Cai[/url], [url=]Xiaojing Dong[/url], [url=]Conghui Wang[/url], [url=]Xia Xiao[/url], [url=]Lili Ren[/url] ... Show more
[url=]Author Notes[/url]
Protein & Cell, pwac013, https://doi.org/10.1093/procel/pwac013
Published:
24 May 2022
[url=]Article history[size=0.5][/url]
AbstractThe emerging of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) caused COVID-19 pandemic. The first case of COVID-19 was reported at early December in 2019 in Wuhan city, China. To examine specific antibodies against SARS-CoV-2 in biological samples before December 2019 would give clues when the epidemic of SARS-CoV-2 might start to circulate in populations. We obtained all 88,517 plasmas from 76,844 blood donors in Wuhan between 1st September and 31st December 2019. We first evaluated the pan-immunoglobin (pan-Ig) against SARS-CoV-2 in 43,850 samples from 32,484 blood donors with suitable sample quality and enough volume. 264 samples from 213 donors were pan-Ig reactive, then further tested IgG and IgM, and validated by neutralizing antibodies against SARS-CoV-2. 213 samples (from 175 donors) were only pan-Ig reactive, 8 (from 4 donors) were pan-Ig and IgG reactive and 43 (from 34 donors) were pan-Ig and IgM reactive. Microneutralization assay showed all negative results. In addition, 213 screened reactive donors were analyzed and didn’t show obviously temporal or regional tendency, but the distribution of age showed a difference compared with all tested donors. Then we reviewed SARS-CoV-2 antibody results from these donors who donated several times from September 2019 to June 2020, partly tested in a previous published study, no one was found a significant increase in S/CO of antibodies against SARS-CoV-2. Our findings showed no SARS-CoV-2 specific antibodies existing among blood donors in Wuhan, China before 2020, indicating no evidence of transmission of COVID-19 before December 2019 in Wuhan, China. [size=1.0625]Issue Section:
Research article
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[size=0.9375]Accepted manuscripts are PDF versions of the author’s final manuscript, as accepted for publication by the journal but prior to copyediting or typesetting. They can be cited using the author(s), article title, journal title, year of online publication, and DOI. They will be replaced by the final typeset articles, which may therefore contain changes. The DOI will remain the same throughout.
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Author notes[size=0.9375]These authors contributed equally to this article.
©The Author(s) 2022. Published by Oxford University Press on behalf of Higher Education Press.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
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